Presenting the latest molecular diagnostic techniques in one comprehensive volume The molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in 2011. With the spread of molecular testing and the development of new technologies and their opportunities, laboratory professionals and physicians more than ever need a resource to help them navigate this rapidly evolving field. Editors David Persing and Fred Tenover have brought together a team of experienced researchers and diagnosticians to update this third edition comprehensively, to present the latest developments in molecular diagnostics in the support of clinical care and of basic and clinical research, including next-generation sequencing and whole-genome analysis. These updates are provided in an easy-to-read format and supported by a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors. Molecular Microbiology: Diagnostic Principles and Practice • Presents the latest basic scientific theory underlying molecular diagnostics • Offers tested and proven applications of molecular diagnostics for the diagnosis of infectious diseases, including point-of-care testing • Illustrates and summarizes key concepts and techniques with detailed figures and tables • Discusses emerging technologies, including the use of molecular typing methods for real-time tracking of infectious outbreaks and antibiotic resistance • Advises on the latest quality control and quality assurance measures • Explores the increasing opportunities and capabilities of information technology Molecular Microbiology: Diagnostic Principles and Practice is a textbook for molecular diagnostics courses that can also be used by anyone involved with diagnostic test selection and interpretation. It is also a useful reference for laboratories and as a continuing education resource for physicians. If you are looking for online access to the latest clinical microbiology content, please visit www.wiley.com/learn/clinmicronow.
Contributors Preface Section I: Novel and Emerging Technologies 1 Nucleic Acid Amplification Methods Overview 2 Application of Identification of Bacteria by DNA Target Sequencing in a Clinical Microbiology Laboratory 3 Microbial Whole-Genome Sequencing: Applications in Clinical Microbiology and Public Health 4 Digital PCR and Its Potential Application to Microbiology 5 Massively Parallel DNA Sequencing and Microbiology 6 Next-Generation Sequencing 7 Pathogen Discovery 8 Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Microbial Identification in Clinical Microbiology 9 Multiplex Technologies Section II: Metagenomics: Implications for Diagnostics 10 The Skin Microbiome: Insights into Potential Impact on Diagnostic Practice 11 The Gastrointestinal Microbiome 12 The Vaginal Microbiome 13 Microbial Communities of the Male Urethra 14 The Human Virome in Health and Disease Section III: Health Care-Associated Infections 15 Molecular Detection of Staphylococcus aureus Colonization and Infection 16 Molecular Diagnostics for Clostridium difficile 17 Overview of Molecular Diagnostics in Multiple-Drug-Resistant Organism Prevention: Focus on Multiple-Drug-Resistant Gram-Negative Bacterial Organisms 18 Detection of Vancomycin-Resistant Enterococci Section IV: Molecular Diagnostics and Public Health 19 The Impact of Molecular Diagnostics on Surveillance of Foodborne Infections 20 Role of Molecular Methods in Improving Public Health Surveillance of Infections Caused by Antimicrobial-Resistant Bacteria in Health Care and Community Settings 21 Molecular Diagnostics: Huge Impact on the Improvement of Public Health in China 22 Surveillance and Epidemiology of Norovirus Infections 23 Molecular Diagnostic Assays for the Detection and Control of Zoonotic Diseases Section V: Syndromic Diagnostics 24 Molecular Approaches to the Diagnosis of Meningitis and Encephalitis 25 Using Nucleic Acid Amplification Techniques in a Syndrome-Oriented Approach: Detection of Respiratory Agents 26 Molecular and Mass Spectrometry Detection and Identification of Causative Agents of Bloodstream Infections 27 Molecular Diagnosis of Gastrointestinal Infections 28 Diagnostic Approaches to Genitourinary Tract Infections 29 Syndromic Diagnostic Approaches to Bone and Joint Infections Section VI: Virology 30 Molecular Detection and Characterization of Human Immunodeficiency Virus Type 1 31 Molecular Detection and Characterization of Hepatitis C Virus 32 Molecular Detection and Characterization of Hepatitis B Virus 33 Molecular Detection of Human Papillomaviruses 34 Molecular Diagnostics for Viral Infections in Transplant Recipients Section VII: Fungi and Protozoa 35 Molecular Detection and Identification of Fungal Pathogens 36 Molecular Approaches for Diagnosis of Chagas' Disease and Genotyping of Trypanosoma cruzi 37 Molecular Approaches for Diagnosis of Malaria and the Characterization of Genetic Markers for Drug Resistance 38 Molecular Detection of Gastrointestinal Parasites Section VIII: Point-of-Care/Near-Care Diagnostics 39 Molecular Diagnostics and the Changing Face of Point-of-Care 40 Point-of-Care Technologies for the Diagnosis of Active Tuberculosis 41 Molecular Diagnostics for Use in HIV/AIDS Care and Treatment in Resource-Limited Settings 42 Rapid Point-of-Care Diagnosis of Malaria and Dengue Infection Section IX: The Host and Host Response 43 Implications of Pharmacogenetics for Antimicrobial Prescribing 44 Exploiting MicroRNA (miRNA) Profiles for Diagnostics 45 Host Response in Human Immunodeficiency Virus Infection 46 Biomarkers of Gastrointestinal Host Responses to Microbial Infections Section X: Information Technology 47 Point-of-Care Medical Device Connectivity: Developing World Landscape 48 WHONET: Software for Surveillance of Infecting Microbes and Their Resistance to Antimicrobial Agents 49 Cloud-Based Surveillance, Connectivity, and Distribution of the GeneXpert Analyzers for Diagnosis of Tuberculosis (TB) and Multiple-Drug-Resistant TB in South Africa Section XI: Quality Assurance 50 Molecular Method Verification 51 Molecular Microbiology Test Quality Assurance and Monitoring 52 Proficiency Testing and External Quality Assessment for Molecular Microbiology 53 Practices of Sequencing Quality Assurance 54 Verification and Validation of Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry-Based Protocols Section XII: The Business of Diagnostics 55 Improved Diagnostics in Microbiology: Developing a Business Case for Hospital Administration 56 Molecular Diagnostics and the Changing Legal Landscape Index
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